Table 2 Deleterious mutations found in all AA1SA isolates
From: A landscape of genomic alterations at the root of a near-untreatable tuberculosis epidemic
Amino acid change | Gene | Product | Function | PROVEAN score* | Essentiality |
|---|---|---|---|---|---|
P251L | ompA (Rv0899) | Outer membrane protein A | Porin of low specific activity | − 7.220 | Essential |
G59D | Rv1877 | Conserved integral membrane protein | Involved in transport of drug across the membrane | − 6.971 | Non-essential |
D53G | cut5b (Rv3724B) | Probable cutinase Cut5b | Hydrolysis of cutin (a polyester that forms the structure of plant cuticle) | − 5.649 | No info |
Q103R | Rv0421c | Conserved hypothetical protein | Unknown | − 2.857 | Non-essential |
R27H | Rv1053c | Hypothetical protein | Unknown | − 5.000 | Non-essential |
P141S | Rv1907c | Hypothetical protein | Unknown | − 3.922 | Non-essential |
A46V | Rv2923c | Conserved protein | Unknown | − 3.437 | Non-essential |
R39W | ruvA (Rv2593c) | Probable Holliday junction DNA helicase | Mediates Holliday junction migration by localised denaturation/reannealing | − 6.219 | Essential |
A381P | ethA (Rv3854c) | Monooxygenase | Activates the pro-drug ethionamide | − 4.576 | Non-essential |
D304N | mprB (Rv0982) | Two-component sensor kinase | Sensor part of a two component regulatory system (MPRAB system) | − 4.284 | Essential |