Fig. 2

Circus plot showing the association between sustained maternal smoking in pregnancy and child blood DNA methylation and transcription along the chromosomes (outer circle). Second circus shows the statistical significance (−log10(p value)) for DNA methylation (dark blue) and transcription (light blue). Only the 18 loci significant at 5% FDR in the methylation analysis are annotated. Next circus shows the direction of the association of the CpGs in these 18 loci with maternal smoking in pregnancy (green, positive; red, inverse; and orange, loci with CpGs associated in both directions). The inner circus shows the 5 loci for which cis eQTMs at 5% FDR were identified (green, positive, meaning higher DNA methylation–higher gene expression; red, inverse; and orange, both). Genes annotated in parenthesis are significant eQTM genes, and none of them corresponds to the closest gene to the CpG site. Loci annotated with an asterisk are those surviving multiple-testing correction only in the any maternal smoking in pregnancy models. To gain graphical resolution, only associations with p value < 0.05 are shown, and p values < 1E−10 are truncated to 1E−10