Fig. 4
From: In utero and childhood exposure to tobacco smoke and multi-layer molecular signatures in children
Regional plot of the AHRR locus (50 kb upstream and downstream of the 5 CpG sites associated with any maternal smoking in pregnancy). The y-axis of the top panel shows the −log (10) p value of the associations between any maternal smoking in pregnancy and methylation levels at CpG sites (circles) and gene expression levels (lines). Only CpGs nominally associated (p value < 0.05) are shown, hyper-methylated in red and hypo-methylated in blue. The top CpG, cg05575921, is shown in purple. Five of the CpGs survived multiple-testing correction (dashed red line): 2 hyper-methylated in intron 1 (cg17924476 and cg23067299), and 3 hypo-methylated at other introns (cg11902777, cg05575921, and cg21161138). The correlation of methylation levels among CpGs is shown at the bottom panel. The middle panel shows the annotation of genes (yellow) and CpG islands (green). The expression of none of the genes in the locus for which there were probes in the gene expression array was associated with any maternal smoking in pregnancy (p value < 0.05). All of them showed negative coefficients of the association (indicated as pink lines). List of TCs and gene annotation: TC05001094.hg.1 annotated to EXOC3-AS1, TC05000006.hg.1 annotated to EXOC3, TC05000005.hg.1 annotated to both AHRR and PDCD6, and TC05002795.hg.1 not annotated