Fig. 5

IGFBP2 protein levels in the lung tissues measured by western blot using IGFBP2 specific antibody or actinin level as a loading control (a). C1–C4 are control donor lung samples, and P1–P4 are lung samples from PAH patients. Box and whisker plot of IGFBP2 protein levels in lung samples from PAH patients versus from controls (b). Boxes represent the interquartile range (IQR 5–95%) and horizontal lines are the median. The IGFBP2 protein level in each sample (bands in a) was quantified using ImageJ software