Fig. 2

Acute effects of cisplatin exposure on germ cell populations in the human foetal testis. AP2γ⁺ gonocytes (brown) and MAGEA4⁺ (pre)spermatogonia (blue) protein expression in the human foetal testis 24 h (a–c) and 96 h (g–i) following exposure to vehicle or cisplatin (0.5 and 1.0 μg/ml). Scale bars represent 100 μm (or 50 μm for insets). Dotted lines outline seminiferous tubules. Quantification of germ cell counts per tubular area (mm²) in the human foetal testicular tissues 24 h (d–f) and 96 h (j–l) following exposure to vehicle (VEH) or cisplatin (0.5 and 1.0 μg/ml). Germ cell numbers were unaffected 24 h post-exposure, whilst a significant reduction in number of gonocytes, (pre)spermatogonia and total germ cells was observed at 96 h following exposure to 0.5 μg/ml and 1.0 μg/ml cisplatin exposure. Data analysed using two-way ANOVA. **p < 0.01, ***p < 0.001 and ****p < 0.0001. Values shown are means ± SEM and each data point represents an individual foetus (n = 9–11)