Skip to main content
Fig. 2 | BMC Medicine

Fig. 2

From: Cisplatin and carboplatin result in similar gonadotoxicity in immature human testis with implications for fertility preservation in childhood cancer

Fig. 2

Acute effects of cisplatin exposure on germ cell populations in the human foetal testis. AP2γ+ gonocytes (brown) and MAGEA4+ (pre)spermatogonia (blue) protein expression in the human foetal testis 24 h (ac) and 96 h (gi) following exposure to vehicle or cisplatin (0.5 and 1.0 μg/ml). Scale bars represent 100 μm (or 50 μm for insets). Dotted lines outline seminiferous tubules. Quantification of germ cell counts per tubular area (mm2) in the human foetal testicular tissues 24 h (df) and 96 h (jl) following exposure to vehicle (VEH) or cisplatin (0.5 and 1.0 μg/ml). Germ cell numbers were unaffected 24 h post-exposure, whilst a significant reduction in number of gonocytes, (pre)spermatogonia and total germ cells was observed at 96 h following exposure to 0.5 μg/ml and 1.0 μg/ml cisplatin exposure. Data analysed using two-way ANOVA. **p < 0.01, ***p < 0.001 and ****p < 0.0001. Values shown are means ± SEM and each data point represents an individual foetus (n = 9–11)

Back to article page