Fig. 4

Acute effects of cisplatin exposure on germ cell proliferation in the human foetal testis. AP2γ⁺ gonocytes (red), MAGEA4⁺ (pre)-spermatogonia (blue) and Ki67 (proliferation; green) protein expression in the human foetal testis 24 h (a, b) and 96 h (f, g) following exposure to vehicle (VEH) or cisplatin (CIS; 0.5 μg/ml). Scale bars represent 100 μm (or 20 μm for insets). Quantification of germ cell counts per tubular area (mm²) in the human foetal testis 24 h (c–e) and 96 h (h–j) following exposure to vehicle or cisplatin (0.5 μg/ml). A decrease in proliferating (pre)spermatogonia and total germ cells was observed at 96 h post exposure to cisplatin. *p < 0.05, **p < 0.01. Data analysed using two-way ANOVA. Values shown are means ± SEM and each set of coloured data points represents an individual foetus (n = 7)