Fig. 5

Long-term effects of cisplatin exposure on human foetal testis xenografts. Immunohistochemistry for AP2γ⁺ gonocytes (red) and MAGEA4⁺ (pre)-spermatogonia (blue) in human foetal testis tissues exposed to vehicle (VEH; a) or cisplatin (CIS; b) for 24 h prior to xenografting (12 weeks). Total graft weight (c) was significantly reduced following cisplatin-exposure. Quantification of cell number (d–f) and proliferation (g–i) of germ cell populations per tubular area (mm²) in xenografts. A significant decrease in all germ cells populations was observed in cisplatin-exposed tissues. Proliferating (pre)spermatogonia and total germ cells were also significantly reduced in cisplatin-exposed tissues after long-term xenografting. Data analysed using two-way ANOVA. *p < 0.05, **p < 0.01 and ***p < 0.001. Values shown are means ± SEM and each set of coloured data points represents an individual foetus (n = 4)