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Fig. 5 | BMC Medicine

Fig. 5

From: Cisplatin and carboplatin result in similar gonadotoxicity in immature human testis with implications for fertility preservation in childhood cancer

Fig. 5

Long-term effects of cisplatin exposure on human foetal testis xenografts. Immunohistochemistry for AP2γ+ gonocytes (red) and MAGEA4+ (pre)-spermatogonia (blue) in human foetal testis tissues exposed to vehicle (VEH; a) or cisplatin (CIS; b) for 24 h prior to xenografting (12 weeks). Total graft weight (c) was significantly reduced following cisplatin-exposure. Quantification of cell number (df) and proliferation (gi) of germ cell populations per tubular area (mm2) in xenografts. A significant decrease in all germ cells populations was observed in cisplatin-exposed tissues. Proliferating (pre)spermatogonia and total germ cells were also significantly reduced in cisplatin-exposed tissues after long-term xenografting. Data analysed using two-way ANOVA. *p < 0.05, **p < 0.01 and ***p < 0.001. Values shown are means ± SEM and each set of coloured data points represents an individual foetus (n = 4)

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