Fig. 3

Identification of hsa_circ_0075451(circGMDS). a Schematic illustration showing the circularization of GMDS exons 2 to 4 forming circGMDS. Red line indicates the special splicing junction of circGMDS. b CircGMDS, along with CDR1as and β-actin, was amplified from cDNA or gDNA from THP-1 cells with divergent and convergent primers. Divergent primers amplified circGMDS and CDR1as in cDNA but not genomic DNA (gDNA). β-Actin was used as a negative control. c qRT-PCR for the abundance of circGMDS and GMDS mRNA in THP-1 cells treated with RNase R. The amount of circGMDS and GMDS mRNA was normalized to the value measured in the mock treatment. d Survival curves of patients with high and low expression of circGMDS in AML patients