Fig. 5

The regulatory axis of hsa_circ_0075451 -| miR-326/miR-330-5p | PRDM16. Hsa_circ_0075451 has putative binding sites of ten miRNAs (a). Dual luciferase reporter assays show evidence of three miRNAs (miR-326, miR-330-5p, and miR-338-3p; a, red bars) interacting with hsa_circ_0075451. Luciferase activity was analyzed in cells cotransfected with each miRNA and hsa_circ_0075451-WT or hsa_circ_0075451-MUT (b–d). The binding sequences of 3 miRNAs and circRNA are illustrated (e). The putative binding sites (f) and sequences (g) of miR-326, miR-330-5p, and miR-338-3p are located in the 3′-UTR of PRDM16. Dual luciferase reporter assays were performed to assess the interaction between miRNAs and PRDM16. Two binding sites of miR-330-5p and one of miR-326 (f, red bars) are validated to exist in 3′-UTR of PRMD16 (h–j).