Fig. 4

Visualization of histone modifications by chromatin immunoprecipitation and DNA sequencing (ChIP-seq) in the human transferrin genomic region. ChIP-seq data was obtained from explants from 5 control livers and 7 livers of patients with severe non-responder alcoholic hepatitis. The genomic region around the transferrin promoter was visualized through the Integrative Genome Viewer (IGV). Histone 3 lysine 4 (H3K4) mono- and tri-methylation as well as H3K27 acetylation and tri-methylation are shown. The decrease in H3K27ac signal indicated the lack of activity in transferrin enhancer and promoter regions, while the decrease of H3K4me3 and increase of H3K27me3 suggested the absence of activity and repressive chromatin configuration, respectively, in the region around the transcription start site (TSS). The light gray areas indicate the regions where these changes were seen (cohort ii)