Table 1 Oligonucleotide primers and PCR conditions of rat and human in real-time quantitative PCR
Genes | Forward primer (5′-3′) | Reverse primer (3′-5′) | Annealing |
|---|---|---|---|
Rat | |||
 GR | CACCCATGATCCTGTCAGTC | AAAGCCTCCCTCTGCTAACC | 61 °C, 30 s |
 FXR | GATGTCTTGGAGGGTGAATG | GAGTGAGACCTGGTACAAATG | 60 °C, 30 s |
 Oatp2b1 | TGTCTGCCGCTACTATGA | CTTGTAGGTCTGAGCTCTTTAC | 60 °C, 30 s |
 Bcrp | GAGCCTTCCAAGAGAGAGA | CTGATGACAGAACGAGGTAAC | 60 °C, 30 s |
 Oatp4a1 | GGTGTGACAACTGGCTATG | GCATAGCAGGGAGAGTAGTA | 60 °C, 30 s |
 Mrp4 | GTGTTGGACAGAGACAGTTAG | TGTGAGCAATGGTGAGAAC | 60 °C, 30 s |
 Cyp7a1 | CCATAAGGTGTTGTGCCACGGAAA | GCCCAAATGCCTTCGCAGAAG | 60 °C, 30 s |
 Cyp27a11 | ACTGCACCAGTTACAGGTGCTTTACA | CCATGTCGTTCCGTACTGGGTACT | 60 °C, 30 s |
 Slc27a5 | GGAGATCACAAACACCTACAA | CCTTGTTGTCCAGTATGTAGAG | 60 °C, 30 s |
 GAPDH | GCAAGTTCAATGGCACAG | GCCAGTAGACTCCACGACA | 60 °C, 30 s |
Human | |||
 GR | GTTACACAGGCTTCAGGTATC | TGGAGTTTCCTTCCCTCTT | 60 °C, 30 s |
 FXR | CCAAAGTCATCTCCTACTTCAG | CAGTGTCTTCCAAGCAGTAG | 60 °C, 30 s |
 OATP2B1 | CCCAGCACTCGTGTGGAATA | GCACGTTGAGTCGCAGGAT | 60 °C, 30 s |
 BCRP | GTCAGAGTGTGGTTTCTGTAG | TGCTGCAAAGCCGTAAA | 60 °C, 30 s |
 OATP4A1 | CTGTATCCCTCAGAATCTTTCC | CATCGTAGAGTTGCCGTTAG | 60 °C, 30 s |
 MRP4 | GTTGGCATTGTGGGAAGA | CAGGTTCCTGAGGTATGATTG | 60 °C, 30 s |
 GAPDH | GGGAAGCTCAAGGGAGATA | CTAAGAGACAAGAGGCAAGAAG | 60 °C, 30 s |