From: Variability of multi-omics profiles in a population-based child cohort
Omics profile | Matrix | Sample size (omics available for both visits) | Number of features | Laboratory processing | Batch correction | Criteria for feature exclusion |
---|---|---|---|---|---|---|
DNA methylation | Blood leukocytes | 149 | 91601 | Randomized by cohort and sex, and panel samples paired in plate and array | Residuals of SVs protecting for cohort, sex and age. Cell type composition also corrected with SVs. | < 98% call rate and < 62.5% ICC |
Gene expression | Whole blood | 105 | 45438 | Randomized by cohort and sex, and panel samples paired in plate. | Residuals of SVs protecting for cohort, sex and age. Cell type composition also corrected with SVs. | < 25% call rate |
miRNA expression | Whole blood | 100 | 453 | Randomized by cohort and sex, and panel samples paired in plate and array. | Residuals of SVs protecting for cohort, sex and age. Cell type composition also corrected with SVs. | < 25% call rate |
Proteins | Plasma | 149 | 36 | Randomized by cohort | Overall protein average minus plate specific protein average subtracted for each individual and each protein | < 30% measurements in the linear range (LIN) |
Serum metabolites | Serum | 154 | 177 | Fully randomized | - | > 30% CV and > 30% BLD + zeros |
Urine metabolites | Urine | 154 | 44 | Fully randomized | - | > 30% CV |