Fig. 2From: The effects of selected inhibitors on human fetal adrenal steroidogenesis differs under basal and ACTH-stimulated conditionsEffects of the selected steroidogenic inhibitors on cell viability and expression of steroid enzyme expression. a Morphology and expression of BrdU+ (proliferation marker) and cPARP+ cells (indicated by arrows, apoptosis marker) investigated on serial sections of fetal adrenal tissue cultured ex vivo with Abiraterone acetate (1 μM), Osilodrostat (1 μM), or Efavirenz (10 μM) for 14 days (a). Counterstaining with Mayer’s hematoxylin. b Triple immunofluorescence with CYP11B1/2 (green), CYP17A1 (orange), CYP21A2 (purple), and DAPI (gray, DNA marker). Age of fetal samples shown (at the start of experiment): vehicle control GW 11 + 3; Abiraterone acetate GW 11 + 3; Osilodrostat GW 11 + 2; Efavirenz GW 9. Scale bars corresponds to 100 μmBack to article page