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Fig. 3 | BMC Medicine

Fig. 3

From: The effects of selected inhibitors on human fetal adrenal steroidogenesis differs under basal and ACTH-stimulated conditions

Fig. 3

Effects of Abiraterone acetate inhibition on classic steroidogenesis in human fetal adrenals. Effects of Abiraterone acetate (1 μM) on HFA tissue cultured ex vivo for 14 days under basal and ACTH-stimulated conditions. a Quantification of androgens, glucocorticoids, and steroidogenic intermediate concentrations presented as a ratio relative to the mean of vehicle controls (indicated by dotted lines). b Affected enzyme activity reflected by CYP17A1 product/substrate ratios of measured steroids relative to the mean of vehicle controls. c Affected CYP17A1 enzyme activity reflected by ratios representing changes in androstenedione/corticosteroids downstream of the mineralocorticoid (corticosterone) and glucocorticoid (cortisol) pathways. Media was pooled from 1 to 13 tissue fragments per treatment depending on the initial size of half of the adrenal gland. Values represent geometric mean with 95% CI, n = 10 (fetuses). Significant difference compared with vehicle controls from the same fetus were based on ln-transformed data using repeated measures one-way ANOVA followed by Tukey’s multiple comparisons test. (*) indicate differences compared with vehicle controls, (¤) indicate differences compared with ACTH treatment. *p < 0.05, **/¤¤p < 0.01, ***/¤¤¤p < 0.001, ****/¤¤¤¤P < 0.0001. DHEAS, dehydroepiandrosterone-sulfate

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