Parameters | Marker selection | Training set | Test set | Independent validation set | |||||
---|---|---|---|---|---|---|---|---|---|
Cancer (n = 24) | Cancer (n = 43) | Control (n = 67) | P value | Cancer (n = 42) | Control (n = 68) | P value | Cancer (n = 83) | Control (n = 98) | |
Age, n (%) | Â | Â | Â | 0.29 | Â | Â | 0.22 | Â | Â |
 ≥ 55 | 23 (96%) | 33 (77%) | 44 (66%) |  | 31 (74%) | 41 (60%) |  | 77 (92.8%) | 57 (58.2%) |
 40–55 | 1 (4%) | 10 (23%) | 23 (34%) |  | 11 (26%) | 27 (40%) |  | 6 (7.2%) | 41 (41.8%) |
Sex, n (%) | Â | Â | Â | 0.002 | Â | Â | 1.00 | Â | Â |
 Female | 6 (25%) | 7 (16%) | 31 (46%) |  | 14 (33%) | 23 (34%) |  | 32 (38.6%) | 52 (53.1%) |
 Male | 18 (75%) | 36 (84%) | 36 (54%) |  | 28 (67%) | 45 (66%) |  | 51 (61.4%) | 46 (46.9%) |
Smoking history, n (%) | Â | Â | Â | 0.02 | Â | Â | 0.44 | Â | Â |
 Smokers | 6 (25%) | 12 (28%) | 7 (10%) |  | 9 (21%) | 10 (15%) |  | 24 (28.9%) | 39 (39.8%) |
 Non-smokers | 18 (75%) | 31 (72%) | 60 (90%) |  | 33 (79%) | 58 (85%) |  | 52 (62.7%) | 59 (60.2%) |
 Unknown |  |  |  |  |  |  |  | 7 (8.4%) |  |
Alcohol history, n (%) | Â | Â | Â | 1.00 | Â | Â | 0.10 | Â | Â |
 Drinkers | 0 | 4 (9%) | 7 (10%) |  | 3 (7%) | 13 (19%) |  | 14 (16.9%) | 48 (49.0%) |
 Non-drinkers | 24 (100%) | 39 (91%) | 60 (90%) |  | 39 (93%) | 55 (81%) |  | 59 (71.1%) | 50 (51.0%) |
 Unknown |  |  |  |  |  |  |  | 10 (12.0%) |  |
Pathological type | Â | Â | Â | Â | Â | Â | Â | Â | Â |
 ESCC, n (%) | 22 (92%) | 39 (91%) |  |  | 38 (90%) |  |  | 72 (86.7%) |  |
 EAC, n (%) | 2 (8%) | 4 (9%) |  |  | 4 (10%) |  |  | 8 (9.6%) |  |
 Others, n (%) |  |  |  |  |  |  |  | 3 (3.6%) |  |
Stage, n (%) | Â | Â | Â | Â | Â | Â | Â | Â | Â |
 Stage 0 | 4 (17%) | 3 (7%) |  |  | 2 (5%) |  |  | 10 (12.0%) |  |
 Stage I | 13 (54%) | 9 (21%) |  |  | 8 (19%) |  |  | 14 (16.9%) |  |
 Stage II | 4 (17%) | 11 (26%) |  |  | 13 (31%) |  |  | 27 (32.5%) |  |
 Stage III | 2 (8%) | 12 (28%) |  |  | 7 (17%) |  |  | 14 (16.9%) |  |
 Stage IV | 1 (4%) | 8 (19%) |  |  | 6 (14%) |  |  | 18 (21.7%) |  |
 Unknown |  |  |  |  |  |  |  |  |  |
Healthy control, n (%) | Â | Â | 62 (93%) | Â | Â | 63 (93%) | Â | Â | 98 (100%) |
Benign disease, n (%) | Â | Â | 5 (7%) | Â | Â | 5 (7%) | Â | Â | Â |