Fig. 3

Testing of the selected methylated DNA and bacterial markers and prediction model construction. A–C Methylation level of ITGA4 (A) and relative abundance of Fn (B) and Pa (C) in fecal samples collected from healthy subjects (N), AA patients and CRC patients. D, E ROC curves along with the corresponding AUCs of ITGA4, Fn, Pa, and FIT in the detection of CRC (D) and AA (E). F ROC curves along with the corresponding AUCs of different combinations of ITGA4, Fn, Pa, and FIT. G Prediction score in normal control and AA and CRC patients generated from the logistic regression model constructed by the combination of ITGA4, Fn, Pa, and FIT and the diagnostic performance of the pd-score using the cutoff value of 0.6 (determined by the Youden index method)