Fig. 1

TBCD effectively detected glioma CTCs in glioma patients. A ROC analysis for the threshold detection of CTCs (AUC= 0.892). B Youden index analysis of TBCD according to previous findings in the group (n=158) with 52 healthy donors and 106 glioma patients. C. Typical CTCs in the peripheral blood leukocytes of the blood sample were visualized using GFP expression. Scale bar, 10 μm. D Images of CTCs in GBM patients revealed by DNA-FISH. Red: 1p; Green: 1q. Scale bar, 50 μm. E Typical CTC images of ImageStream® flow cytometry analysis. Ch01: bright field; Ch02: GFP (green); Ch07: CD45 (purple); Ch11: CD56 (red). CTCs were identified as CD45⁻/GFP⁺/CD56⁺ or CD45⁻/GFP⁺/CD56⁻, and WBCs were CD45⁺/CD56⁺. Scale bar, 10 μm. F Number of CTCs in 6 pathologic types of gliomas. A, astrocytoma; O, oligodendroglioma; AO, anaplastic oligodendroglioma; OA, oligoastrocytoma; AOA, anaplastic oligoastrocytoma; GBM, glioblastoma multiforme. G Comparison of CTC counts in grade II (n=36), grade III (n=26), and grade IV (n=26) gliomas. H Comparison of CTC counts between low-grade (WHO grades 1 and 2, n=38) and high-grade (WHO grades 3 and 4, n=52) gliomas. I The number of CTCs detected in preoperative and postoperative patients. ****, p< 0.0001. J The number of CTCs detected in the low-grade group and the high-grade group. *, p=0.044; ****, p< 0.0001