Fig. 6

Gene profiles positively correlated with CTC count. A The 10 most significant Gene Ontology (GO) terms enriched in the genes of interest. GeneRatio is the percentage of total differential genes in the given GO term. Count is the number of total differential genes in the given GO term. B The maximal subnetwork constructed with the genes of interest. The pink nodes represent neutrophil activation, and the second-largest brown node is neutrophil degranulation. The size and color of the edges indicate the coexpression values. Count is the number of total differential genes in the given GO term. C. GSEA of glioma sample data (n=31) for the c7 immunological gene sets downloaded from the Molecular Signatures Database (MSigDB). GeneRatio is the percentage of total differential genes in the given GO term. Count is the number of total differential genes in the given GO term. D. GSEA of glioma sample data (n=31) using the hallmark v7.0 gene sets (MSigDB). GeneRatio is the percentage of total differential genes in the given GO term. Count is the number of total differential genes in the given GO term. E GSEA of the peripheral blood leukocyte RNA-seq dataset. Activated gene sets showed TNF-α signaling via NF-κB (p value< 0.05) with a minimum enrichment score of 0.3 (upper image). Activated gene sets showed TNF-α signaling via hypoxia (p value< 0.05) with a minimum enrichment score of 0.3 (bottom image). F The relationship between NET scores and gene sets in the CTC-positive and CTC-negative groups. G A volcano plot of NET-related differential genes was obtained between the CTC-positive group and the CTC-negative group