Fig. 2
CAR-T cells showed stronger antitumor activity in vivo after SFN pre-treatment. A Analysis of specific lysis of tumor cells. H322 cells were incubated with meso CAR-T cells at various effector to target (E:T) ratios (1:1, 5:1, and 10:1) for 6 h, and lysis of H322 cells was analyzed by flow cytometry. B The expression of PD-1 on SFN-treated CD8+ meso CAR-T cells was analyzed by flow cytometry. C After co-culturing with H322 cells at a 1:1 ratio for 24 h, the secretion of IFN-γ by CD8+ meso CAR-T cells was investigated by flow cytometry. D, E NOD/SCID mice were injected with 5 × 105 H322-luc cells. After 5 days, mice were divided into three groups randomly and were infused with 5 × 106 T cells (i.v.). Bioluminescence images of five representative mice in the three treatment groups are shown after adoptive therapies (D). The bioluminescence signal was measured at different time points (E). F Kaplan-Meier survival curves are presented. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001 (Student’s t test)