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Fig. 4 | BMC Medicine

Fig. 4

From: Sulforaphane enhances the antitumor response of chimeric antigen receptor T cells by regulating PD-1/PD-L1 pathway

Fig. 4

PD-L1 was downregulated by SFN in a poly-ubiquitination dependent manner. A PD-L1 expression (count) according to RNA-seq data. B Analysis of PD-L1 expression on A549 cells at the transcriptional level in three groups (control, IFN-γ, and IFN-γ + SFN) by qRT-PCR. C Schematic representation of the bioinformatics analysis process. D Gene set enrichment analysis (GSEA) analysis of whole-gene counts from RNA-seq data. E The core enrichment genes enriched in ubiquitination-mediated proteolysis pathway were ranked by Rank Metric Score and p value. F List of genes with scores less than − 1 and p values less than 1e−10. G Analysis of BTRC expression (count) from RNA-seq data. H–J Analysis of BTRC (β-TrCP) expression in the control, IFN-γ, and IFN-γ + SFN groups by qRT-PCR and western blot in A549 and H322 cells. K–M Analysis of the transfection efficacy of BTRC siRNA in A549 and H322 cells by qRT-PCR and western blot. N PD-L1 expression was detected in the presence of IFN-γ or SFN after transfection with si-Scr or si-BTRC by western blot on A549 and H322 cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant (Student’s t test)

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