Fig. 4
From: P-gp expression inhibition mediates placental glucocorticoid barrier opening and fetal weight loss
Effects of caffeine (0–100 μM) on P-glycoprotein (P-gp) expression, cortisol transport, and related pathway in placental trophoblasts. A, B Relative mRNA (n=6) and protein (n=3) expression levels of P-gp in primary placental trophoblasts; C, D relative mRNA (n=6) and protein (n=3) expression levels of P-gp in BeWo cells; E Rho 123 accumulation was analyzed by the fluorescence plate reader, n=6; F Effect on cortisol efflux in BeWo cells by caffeine treatment, n=6; G relative mRNA expression levels of RYRs, n=6; H protein levels of JNK, p-JNK, and E1A-binding protein P300 (P300), n=3; I Protein-protein interactions between p-JNK and YB-1 and between YB-1 and P300; J Relative protein levels of the nucleus and cytosol YB-1, n=3; K binding level of YB-1 on the ABCB1 promoter region; binding level of YB-1 and P300 on the ABCB1 promoter region; L histone 3 Lysine 9 (H3K9) and histone 3 Lysine 14 (H3K14) acetylation levels on the ABCB1 promoter region, n=3. Mean ± S.E.M. *P<0.05, **P<0.01 vs. control