Fig. 5
From: P-gp expression inhibition mediates placental glucocorticoid barrier opening and fetal weight loss
Effects of dantrolene, SP600125, and Y-box protein 1 (YB-1) overexpression on P-glycoprotein (P-gp) expression and cortisol efflux function in BeWo cells. A, B Relative mRNA expression levels of ATP-binding cassette, sub-family B, member 1 (ABCB1), and protein levels of C-Jun N-terminal kinase (JNK)/p-JNK treating with 10 μM caffeine and/or 10 μM dantrolene; C effect of caffeine and/or dantrolene on cortisol efflux, n=6; D–F relative protein levels of JNK/p-JNK, nucleus YB-1, and relative mRNA expression level of ABCB1 after treatment with 10 μM caffeine and/or 10 μM SP600125; G effect of caffeine and/or SP600125 on cortisol efflux, n=6; H relative YB-1 protein level after transiently transfected with YB-1 plasmid or control empty plasmid; I relative mRNA expression of YB-1 after treatment with 10 μM caffeine and/or YB-1 transfection, n=6; J histone 3 Lysine 9 (H3K9) and histone 3 Lysine 9 (H3K14) acetylation changes on ABCB1 promoter after YB-1 overexpression, n=3; K, L effect of caffeine and/or YB-1 overexpression on P-gp protein expression (n=3) and cortisol efflux (n=6). Mean ± S.E.M. *P<0.05, **P<0.01 vs. control; #P<0.05, ##P<0.01 vs. 10 μM caffeine