Table 1 Oligonucleotide primers in quantitative real-time PCR
From: P-gp expression inhibition mediates placental glucocorticoid barrier opening and fetal weight loss
Gene | Forward primer | Reverse primer | Locus |
|---|---|---|---|
Rat | |||
 abcb1a | TAGCAGGAGTGGTTGAAATG | CAAGCTCTGGGCATACATAG | NM_133401.2 |
 abcb1b | CATCCAGAACGCAGACTTGA | CAGCCTGAACCATCGAGAAA | NM_012623.3 |
 RYR1 | CATCCTTTCATCCGTCACTC | TCATCTTCGCTCTTGTTGTAG | XM_039100851.1 |
 RYR2 | AGGGAGAGAGGAAGCCATTA | GGTCACTGAGACCAGCATTT | XM_039096071.1 |
 RYR3 | CACTGACAACTCCTTTCTCTAC | GGATCGTCCTCAAGGTCTTA | XM_039106806.1 |
 GAPDH | GCAAGTTCAACGGCACAG | GCCAGTAGACTCCACGACA | NM_017008.4 |
Mouse | |||
 abcb1a | CAGCCAGCATTCTCCGTAATA | GTGAGGATCTCTCCAGCTTTG | NM_011076.3 |
 abcb1b | TCCCTGTTCTTTCTGGTTATGG | CCCGAGGTTTGCTACATTCT | NM_011075.2 |
 UBC | AGGTGGGATGCAGATCTTTG | CCTCCTTGTCCTGGATCTTTG | NM_019639.4 |
Human | |||
 ABCB1 | GGTGGTGTCACAGGAAGAGATT | TCTAACAAGGGCACGAGCTATG | NM_001348945.2 |
 RYR1 | GCTCCCTGTGTGTGTGTAAT | CGGATGCTGGTGACATAGTT | NM_000540.3 |
 RYR2 | GAGATGGTCCCTCACCAAATAG | CGTCCCAAGAGGTCAATCAA | NM_001035.3 |
 RYR3 | GGAGAAGGTCAGCATAGACAAG | TCCAGTCACCACTTCAAACTC | NM_001036.6 |
 YB-1 | GCAGCAGACCGTAACCATTAT | TCTCCGATCCCTCGTTCTTT | NM_004559.5 |
 P300 | CCAGCCATGCAGAACATGAA | CGGAATTGTGAAGGCATGGT | NM_001429.4 |
 GAPDH | GAAATCCCATCACCATCTTCCAG | ATGAGTCCTTCCACGATACCAAAG | NM_002046.7 |