Fig. 1From: Upregulation of NOD1 and NOD2 contribute to cancer progression through the positive regulation of tumorigenicity and metastasis in human squamous cervical cancerNOD1 and NOD2 are upregulated in human CSCC tissues and associated with poor survival. A Partial heat map showing differentially expressed NLR genes including NOD1 and NOD2 in the CSCC (n = 4) and normal cervix (n = 6) tissues. B NOD1 and NOD2 mRNA copy numbers in unpaired CSCC tissues (NOD1, n = 59; NOD2, n = 24) and normal cervix (NOD1, n = 33; NOD2, n = 31). C NOD1 and NOD2 mRNA expression in the CSCC (NOD1, n = 75; NOD2 n = 75) and normal cervix samples (non-tumoral adjacent tissue, NOD1, n = 188; NOD2 n = 188) extracted from TCGA database. D Representative immunofluorescence images showing co-staining of AE1/AE3 and NOD1/NOD2 in paired CSCC tumors and normal cervix tissues (data were from two independent experiments with eight samples). E Representative IHC images showing in situ expression of NOD1and NOD2 in paired human CSCC tissues of different pathological stages (early and late stages and LVSI) and adjacent non-tumor tissues (scale bar = 100 μm and magnification—× 10 or × 20). F–H IHC scores of NOD1 and NOD2 in F paired tumor and adjacent non-tumor tissues (NOD1, n = 75; NOD2, n = 70), G tumors with and without LVSI (NOD1: LVSI = 58, non-LVSI = 45; NOD2: LVSI = 48, non-LVSI = 49), and H tumors with and without LM (NOD1: LM = 48, non-LM = 48; NOD2: LM = 55, non-LM = 39). I Kaplan-Meier curves showing overall survival of CSCC patients demarcated on the basis of in situ NOD1 expression (http://www.proteinatlas.org). J NOD1 and NOD2 mRNA levels and representative immunofluorescence images showing respective protein levels in Siha and CasKi cell lines. K Immunofluorescence images showing respective NOD1 and NOD2 protein levels in primary CSC cells. For cell lines, the experiments were performed in two wells with three replicates; for primary cells, the experiments were performed by two independent experiments with four samples; the picture is a representative one. *P < 0.05, ** P < 0.01, *** P < 0.001Back to article page