Skip to main content
Fig. 3 | BMC Medicine

Fig. 3

From: Functional genomics elucidates regulatory mechanisms of Parkinson’s disease-associated variants

Fig. 3

Validation of the regulatory effects of the TF binding-disrupting SNPs with dual luciferase reporter gene assays. Six TF binding-disrupting SNPs showed significant differences between different alleles, with the same allelic effect direction in both SH-SY5Y and U251 cells. a The constructs containing the C allele of rs3735901 exhibited significantly higher luciferase activity than the construct containing the T allele in SH-SY5Y and U251 cells. b The C allele of rs11136093 conferred significantly higher luciferase activity than the T allele in SH-SY5Y and U251 cells. c The reporter vectors containing the G allele of rs62061727 displayed significantly higher luciferase activity than the T allele in SH-SY5Y and U251 cells. d The constructs carrying the T allele of rs62061809 exhibited significant higher luciferase activity than the constructs carrying the C allele in SH-SY5Y and U251 cells. e The reporter vectors containing the T allele of rs62064663 showed significantly higher luciferase activity than the G allele in SH-SY5Y and U251 cells. f The reporter vectors containing the single base deletion of rs143191191 showed significantly higher luciferase activity than the A allele in SH-SY5Y and U251 cells. N = 8 for the control group, n = 16 per experimental group for SH-SY5Y and U251 cells. Two-tailed Student’s t test was used for statistical analyses. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page