Fig. 4

Disruption of REST and SIN3A binding by SNP rs6781790. a,b rs6781790 disrupts the binding of REST and SIN3A TFs. c The 1 kb genomic sequence surrounding SNP rs6781790 was displayed with DNase-Seq signal (light blue), the transcription factor (TF) chromatin immunoprecipitation, and sequencing (ChIP-Seq) signal (green), and histone modifications (purple). d Reporter gene assays showed that the T allele of rs6781790 conferred significantly higher luciferase activity than the C allele in SH-SY5Y and U251 cells. e Allele-specific expression (ASE) analysis showed that different alleles of rs6781790 exhibited significant preferential expression in human brain tissues. f–i SIN3A knockdown resulted in significant downregulation of GPX1, P4HTM, and WDR6, indicating that these genes are regulated by the SIN3A. N = 8 for the control group, n = 16 per experimental group in SH-SY5Y and U251 cells. n = 3 per group in f–i. Two-tailed Student’s t test was used for statistical analyses. **P < 0.01, ***P < 0.001