Fig. 6

TP deficiency causes oxidative stress and cellular senescence in ASC cells. Data were obtained at D0 in undifferentiated ASC, ASC with a CRISPR-Cas9-mediated TP-knockout (KO), and ASC with a Cas9/scramble gRNA KO corresponding to control (CTL) cells. All results are expressed as means ± SEM of five independent experiments. A Representative images of senescence-associated β-galactosidase activity (SA-β-gal) after staining at pH4 and pH6. Scale bar is 100 μm. The SA-β-gal staining ratio at pH 6.0/pH 4.0 was calculated. ***p < 0.001. B Evaluation of cellular senescence by Western blotting using antibodies against the indicated proteins. Numbers on the left correspond to molecular weight markers (kDa)