Fig. 1

Flowchart for sample selection, mapping and filtering of dual RNA-seq for the generation of gene expression matrices for S. pyogenes monomicrobial infections (Hstrep -Human, Bstrep -Bacteria) and polymicrobial infections (Hpoly – Human, Bpoly -Bacteria). Bacterial and Human gene expression are measured on the same tissue biopsies from NSTI patients. Clinical information associated with patients and samples\biopsies is given in Table 1