Fig. 2

Examples and schematic diagrams of exonic-breakpoint fusions generating no chimeric fusion transcripts. A DNA NGS detected an exonic-breakpoint ALK fusion involving EML4 exon 21 and ALK intron 19 in P1. B Schematic diagram of the predicted fusion detected by DNA NGS but not by RNA NGS due to open reading frame disruption. C DNA NGS detected an uncommon exonic-breakpoint RET fusion involving ADAMTS2 intron 10 and RET exon 3 in P6. D Schematic diagram of the predicted fusion detected by DNA NGS but not by RNA NGS due to different transcriptional orientations (5’-5’ fusion). E DNA NGS detected an uncommon exonic-breakpoint ROS1 fusion involving NKAIN2 intron 6 and ROS1 exon 35 in P10. F Schematic diagram of the predicted fusion detected by DNA NGS but not by RNA NGS due to different transcriptional orientations (3’-3’ fusion). The gray bars indicate sequencing reads that match the reference genome, and multicolored bars indicate mismatched reads (the corresponding partners). The asterisk indicates a premature stop codon. The rectangles indicate exons (E, exon), and the solid lines indicate introns. The arrows indicate the direction of transcription