Fig. 1

Transient treatment with Nutlin-3 has a long-lasting rescue effect on impaired hippocampal neurogenesis in FMR1-deficient mice. a Experimental scheme for assessing the hippocampal neurogenesis in Fmr1 KO and WT mice treated with Nutlin-3 (Nut3) or vehicle (Veh). b Sample confocal images used for identifying NSCs (GFP+GFAP+) and proliferating NSCs (GFP+GFAP+MCM2+) in the DG of adult Fmr1 KO and WT mice bred into a Nestin-GFP mouse background. Scale bar, 20 μm. c Comparison of the percentage of activated NSCs among total NSCs in the DG of Fmr1 KO and WT mice with or without Nutlin-3 treatment (n = 3 or 4 per group). d Sample confocal images to identify new mature neurons (NeuN+BrdU+) in the dentate gyrus of Fmr1 KO and WT mice. Scale bar, 20 μm. e Comparison of the percentage of mature neurons among BrdU+ cells in DG of Fmr1 KO and WT mice with or without Nutlin-3 treatment (n = 3 per group). *P < 0.05; **P < 0.01; ***P < 0.001. Data are presented as means ± SEM