Fig. 1

Distinct distribution of EZH2 and ADAM12 in human placental villi. A, B Immunofluorescent staining showed that EZH2 (red) was localized mainly in the cytotrophoblast (CTB) but not the syncytiotrophoblast (STB) which was stained for 11β-HSD2 (green) at both early and term pregnancies. Nuclei were counterstained blue with DAPI. Immunohistochemical staining revealed a similar distribution pattern of EZH2. C, D Immunofluorescent staining showed that ADAM12 (green) was mainly localized in the syncytiotrophoblast (STB) and to a much lesser extent in the cytotrophoblast (CTB) at both early and term pregnancies. Nuclei were counterstained blue with DAPI. Immunohistochemical staining revealed a similar distribution pattern ADAM12. Non-immune serum served as negative control (NC). Scale bars, 20 μm