Fig. 4

Role of STAT5B in the regulation of ADAM12-S expression during syncytialization of human trophoblasts. A–C siRNA-mediated knockdown of STAT5B expression increased the abundance of ADAM12S mRNA (n = 4) and protein in cultured trophoblasts (n = 6) as well as secreted ADAM12-S in trophoblast culture medium (n = 3). Randomly scrambled siRNA served as negative control (NC). Top panels of A–C are representative immunoblots. D Increased enrichment of STAT5B at ADAM12 promoter upon syncytialization of cultured trophoblast (n = 4). Top panel of D illustrates aligning positions of primers used in ChIP assay. TSS, transcription start site. Data are means ± SEM. *P < 0.05, ***P < 0.001 vs NC or 3 hrs