Fig. 5

Role of EGF/STAT5B pathway in the induction of ADAM12-S expression during syncytialization of human trophoblast. A Increased EGFR mRNA and protein abundance during syncytialization (n = 4). B Time course of EGF (10 ng/mL) on STAT5B phosphorylation in syncytiotrophoblasts (n = 4). C Effects of EGF (10 ng/mL, 24 hrs) on STAT5B mRNA (n = 3) and protein abundance in syncytiotrophoblasts (n = 4). D siRNA-mediated knockdown of STAT5B attenuated basal and EGF (10 ng/mL)-induced ADAM12S mRNA expression (n = 5) and ADAM12-S secretion (n = 5) in cultured trophoblasts. Randomly scrambled siRNA served as negative control (NC). Top panel of each figure is the representative immunoblot. Data are means ± SEM. *P < 0.05, **P < 0.01 vs corresponding control; ^##P < 0.01 vs EGF-treated group