Fig. 1

The four main aspects of our analysis (A–D). A Using paired t-tests, we compared levels/abundance (log2 RFUs) of proteins between the uterine vein (UV) and radial artery (RA) in 4-vessel samples to define placental uptake and release on the maternal side of the placenta. B A machine learning algorithm (the variable selector elastic net with stability selection) identified placenta-derived proteins that can predict gestational age, a “placental proteomic clock.” C We performed another set of comparisons to define placenta-specific released proteins (AV = antecubital vein). D Placenta-specific released proteins (based on C) were further characterized by the gestational changes in maternal protein levels (log2 RFU) using samples from the STORK cohort. Created with BioRender.com