Fig. 5
EGABA is hyperpolarized in CA1 pyramidal neurons of rats after neonatal severe inflammation. (A) Schematic illustrating the chronological order used for siRNA delivery, LPS administration and perforated patch recordings. Fifteen litters were used in this cohort of experiment. (B) Representative traces of GABA-induced currents to the holding potential from –80 to –30 mV in 10 mV increments of hippocampal neurons at P7-P10. (C) Current–voltage (I-V) curve of GABA-induced currents recorded at different holding potentials from − 80 to − 30 mV in 10 mV increments of pyramidal neurons at P7-P10. (D) EGABA values per cell obtained from all I-V curves indicating a hyperpolarizing shift in septic rats at P7-P10 (n = 10–12 cells from 4–5 rats). (E) RMP values showing a hyperpolarizing shift in septic rats at P7-10 (n = 9–14 cells from 5–6 rats). (F) Representative traces of GABA-induced currents to the holding potential from –80 to –30 mV in 10 mV increments of hippocampal neurons at P14-P16. (G) Current–voltage (I-V) curve of GABA-induced currents recorded at different holding potentials from − 80 to − 30 mV in 10 mV increments of pyramidal neurons at P14. (H) EGABA values per cell obtained from all I-V curves indicating a hyperpolarizing shift in septic rats at P14-P16 (n = 7 cells from 3–4 rats). (I) RMP values showing a hyperpolarizing shift in septic rats at P14-P16 (n = 10–13 cells from 4–5 rats). (J) Current–voltage (I-V) curve of spontaneous GABA-induced currents recorded at different holding potentials from − 80 to − 30 mV in 10 mV increments of pyramidal neurons at P28-P32. (K) EGABA values per cell obtained from all I-V curves indicating a hyperpolarizing shift in septic rats at P28-32 (n = 6–7 cells from 3–4 rats). (L) RMP values showing a hyperpolarizing shift in septic rats at P28-P32 (n = 8–10 cells from 4–5 rats). (M) Representative traces of GABA-induced currents to the holding potential from − 80 to − 30 mV in 10 mV increments of hippocampal neurons at P7-P10 after siRNA injection. (N) Current–voltage (I-V) curve of GABA-induced currents recorded at different holding potentials from − 80 to − 30 mV in 10 mV increments of pyramidal neurons at P7-P10 after siRNA injection. (O) EGABA values per cell obtained from all I-V curves at P7-P10 after siRNA injection (n = 7–8 cells from 3–4 rats). (P) RMP values at P7-P10 after siRNA injection (n = 7–11 cells from 4–5 rats). (Q) Current–voltage (I-V) curve of GABA-induced currents recorded at different holding potentials from − 80 to − 30 mV in 10 mV increments of pyramidal neurons at P14-P16 after siRNA injection. (R) EGABA values per cell obtained from all I-V curves at P14-P16 after siRNA injection (n = 5–7 cells from 3–4 rats). (S) RMP values at P14-P16 after siRNA injection (n = 7–9 cells from 3–4 rats). LPS: lipopolysaccharide; NS: normal saline; EGABA: GABA reversal potential; Panels D, E, H, I, K, and L were compared by unpaired two-tailed Student’s t test; Panels O, P, R, and S were compared by one-way ANOVA with repeated measures followed by a Tukey post hoc test; * P < 0.05, ** P < 0.01, and *** P < 0.001, n.s.: no significance; Error bars indicate SD