Fig. 6

A bidirectional crosstalk occurs between PPAT explants and PCa cell lines. PCa cell lines (PC-3 or LNCaP) were co-cultivated with PPAT explants for 48 h and expression of lipid-, inflammatory-, and tumor-related genes were evaluated by RT-qPCR. A Co-cultured explants with LNCaP or PC-3 cells. B Co-cultured PC-3 cells. C Co-cultured LNCaP cells. D Uptake of the fatty acid analog TF2-C12 at 30 and 60 min in PCa cell lines co-cultured with PPAT explants. E Lipid accumulation measured with Nile Red dye after 15-min incubation in PCa cell lines co-cultured with PPAT explants. Different lettering over the boxes indicates statistical differences. Significant differences are established at p < 0.05. Data are expressed as mean ± SEM (n = 6 experiments)