Fig. 3

Cytotoxicity effect of SCF-DM1 on GIST cell lines. A Cell proliferation assay of GIST cells with treatment of drugs. B FACS detection of apoptosis in GIST cells with treatment of drugs. C Immunoblot analysis of apoptosis-related proteins of GIST cells with treatment of drugs. Lane 1: control; lane 2: SCF-DM1 25 nM; lane 3: SCF-DM1 50 nM; lane 4: DM1 250 nM; lane 5: DM1 500 nM; lane 6: imatinib 500 nM. D Immunoblot analysis of p53 and p21 protein in GIST cells with treatment of SCF-DM1. E Total MDM2 expression of GIST 430 cells with treatment of drugs for 72 h. F Cell cycle analysis of GIST cells with treatment of drugs. Numbers indicated G₀/G₁ or G₂/M population (%). SCF-DM1: 50 nM, DM1: 200 nM. G Clonogenic assay of GIST cells with treatment of drugs. SCF-DM1: 50 nM; imatinib: 500 nM for GIST T1, 1000 nM for GIST 430