Fig. 2

Dapagliflozin inhibits the expression of profibrotic proteins SGK1 and ENaC assessed by confocal immunofluorescence microscopy. A Comparison of SGK1 (green) expression in myocardial tissue. B Comparison of ENaC (green) expression in myocardial tissue. Relative area measurements were determined using a Zeiss LSM 700. Scale bar = 50μm. n = 3 each slide of 3 sections. C The direct effects of dapagliflozin on immunohistochemistry analysis of NHE1 in myocardial tissue (n = 10 per group). Scale bar = 200μm. D RT-PCR analyses of indicated genes in myocardial tissue (n = 10 per group). E Western blot analyses of indicated genes in myocardial tissue (n = 4 per group). Values are means ± SEM. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 compared to control group, ^†p < 0.05, ^††p < 0.01 compared to diabetes group