Fig. 4

Dapagliflozin for suppression of fibrosis via SGK1, ENaC, and NHE1. A Representative RT-PCR bands of SGK1 in H9C2 transfected with SGK1 10-30 nM siRNA. B Representative Western blot bands of SGK1 in H9C2 transfected with SGK1 10-30 nM siRNA. Values are means ± SEM (n = 4). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 compared to the siCon group. C RT-PCR analyses of indicated genes in H9C2. D Western blot analyses of indicated genes in H9C2. Values are means ± SEM (n = 6). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 compared to the siCon group, ^†p < 0.05, ^††p < 0.01, ^†††p < 0.001 compared to palmitic acid + LPS siCon, ^∥p < 0.05 compared to palmitic acid + LPS siSGK + dapa, ^§p < 0.05, ^§§p < 0.01, ^§§§p < 0.001 compared to palmitic acid + LPS HG + siCon, ^¶p < 0.05 compared to palmitic acid + LPS HG + siSGK + dapa