Fig. 5

Protective effect of dapagliflozin for mitochondrial cristae in myocardial tissue. Comparison of mitochondrion changes. A Transmission electron microscopy images of myocardial tissue treated with dapagliflozin. Scale bar = 5μm, 2μm, and 500nm from low to high magnification. B Immunohistochemistry analysis of Fis-1 and Mfn-1 expression and quantification of myocardial tissue cross-sectional area. Scale bar = 200μm. C RT-PCR analyses of indicated genes in myocardial tissue. D Western blot analyses of indicated genes in myocardial tissue (n = 4 per group). Values are means ± SEM (n = 10 per group). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 compared to the Control group, ^†p < 0.05 compared to the diabetes group