Fig. 3

EMT phenotype in tubal secretory cells and fibroblast. A The similarities between 4 major secretory cell subtypes and our epithelial cell clusters, the width of line describes the value of similarity scores. B Heatmap shows the expression level of secretory cell subtype signatures in all secretory cells from six samples, and the EMT signature highly expressed in 126 single cells (indicated by red box) from one BRCA1 mutation carrier. C IHC staining confirms the existence of EMT cluster by its markers SFRP4 in epithelial and stromal compartments of a tubal section from a BRCA1 carrier (BRCA1_1) and D non-BRCA1 carrier (Normal_3). E, F Expression levels of EMT-related transcriptional factors ZEB1 and ZEB2 in epithelial cell clusters. G The distribution of EMT-TF in fibroblast of BRCA1 mutation carriers and normal controls, heatmap shows the ratio of observed cell number over the expected cell number of EMT-TF expressed cells, the dots describe the adjusted p value (FDR) of chi-squared test, which were estimated using the Benjamini-Hochberg procedure