Fig. 4
From: LncRNA Airn maintains LSEC differentiation to alleviate liver fibrosis via the KLF2-eNOS-sGC pathway
Airn maintained LSEC differentiation in vitro. A Primary LSECs were transfected with siAirn-1, siAirn-2, , 20μm. D Primary LSECs were infected with LV-Airn and LV-Control for 72 h. The RNA level of Airn, Vefgr2, eNos, Lyve-1, VE-cadherin, Angpt-2, and Laminin was detected by qRT-PCR. E The protein level of VEGFR2 and eNOS was determined by western blot and quantitatively compared with GAPDH as a reference control. F The expression of VE-Cadherin and LAMININ was determined by confocal microscopy and quantitatively compared. DAPI-stained nuclei blue; scale bar, 20 μm. The data are expressed as the mean ± SD for at least triplicate experiments, *p<0.05 stands for vs siRNA-Control or LV-Control