Fig. 7

Airn maintained LSEC differentiation through KLF2-eNOS-sGC pathway. Primary LSEC were transfected with siAirn or siRNA-Control for 48 h and further treated with 30 μM YC-1 for additional 24 h. A The expression of Lyve-1, Laminin, Wnt9b, HGF, and Wnt2a was detected by qRT-PCR. B The expression of LAMININ was detected by confocal microscopy and quantitatively compared. DAPI-stained nuclei blue; scale bar, 20 μm. C–E Primary LSEC were transfected with siAirn or siRNA-Control for 48 h, the expression of KLF2 was detected by qRT-PCR (C), confocal microscopy (D) and quantitatively compared. DAPI-stained nuclei blue; scale bar, 20 μm. E Primary LSEC were transfected with LV-Airn or LV-Control for 48 h and further treated with siKLF2 for additional 48 h. The expression of eNos and Laminin was detected by qRT-PCR. All data are presented as means ± SD for at least triplicate experiments. F ChIP analyses were performed on indicated gene promoter regions using anti-EZH2 antibody in the single-cell suspensions of mouse liver. G AML12 cells were infected with LV-Airn or LV-Control, and ChIP analyses were performed on indicated genes promoter regions using anti-EZH2 antibody. *p < 0.05 stands for vs siRNA-Control or LV-Control + NC