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Fig. 2 | BMC Medicine

Fig. 2

From: Identification of a window of androgen sensitivity for somatic cell function in human fetal testis cultured ex vivo

Fig. 2

Manipulation of steroidogenesis in ex vivo culture of human fetal testes from gestational weeks 7–21. Quantification of A 17-OHP, B progesterone, C 11-deoxycortisol and D cortisone produced in the fetal testis tissue ex vivo cultures and secreted to the media droplets following treatment with ketoconazole (10−6 M) and hCG (1 IU/ml) for 14 days. Media were collected every 48 h throughout the 14-day culture period and were pooled for each individual tissue piece. Steroid metabolites were measured by LC-MS/MS and are shown as ratios compared to the mean of the corresponding vehicle controls (from the same fetus). Samples are divided into four groups according to developmental timepoints corresponding to gestational weeks (GW) 7–10, 10–12, 12–16 and 16–21. Values represent mean ± SEM, with N=5–6 for each age group and treatment. Significant difference compared to vehicle control, ****p<0.0001, ***p<0.001, **p<0.01 and *p<0.05

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