Fig. 4
Effects of manipulating androgen production in ex vivo cultured human fetal testis tissue on Sertoli cell function. Quantification of A AMH and B Inhibin B secretion from the Sertoli cells by the ex vivo cultured fetal testis tissue following treatment with ketoconazole (10−6 M) and hCG (1 IU/ml) for 14 days. Media were collected every 48 h throughout the 14-day culture period and were pooled for each individual tissue piece. AMH and Inhibin B were measured by ELISA and are shown as ratios compared to the mean of the corresponding vehicle controls (from the same fetus). Samples are divided into four groups according to developmental timepoints corresponding to gestational weeks (GW) 7–10, 10–12, 12–16 and 16–21. Values represent mean ± SEM, with N=5–6 for each age group and treatment. Significant difference compared to vehicle control, ****p<0.0001, ***p<0.001, **p<0.01 and *p<0.05