Fig. 7

Effects of manipulating androgen production in ex vivo cultured human fetal testis tissue on germ cell density. Quantification of A gonocytes, B pre-spermatogonia and C total germ cell number per mm² (density) in the ex vivo cultured fetal testis tissue following treatment with ketoconazole (10⁻⁶ M) and hCG (1 IU/ml) for 14 days. Gonocytes were determined as the number of OCT4⁺ cells per mm², pre-spermatogonia as the number of MAGE-A4⁺ cells per mm² and total germ cell number as the combined number of OCT4⁺ and MAGE-A4⁺ cells per mm². Samples are divided into four groups according to developmental timepoints corresponding to gestational weeks (GW) 7–10, 10–12, 12–16 and 16–21. Values represent mean ± SEM, with N=5–6 for each age group and treatment. Significant difference compared to vehicle control, *p<0.05