Fig. 9

Effects of reduced androgen exposure via blocking of the androgen receptor in ex vivo culture of human fetal testes on germ cell density. Quantification of A gonocytes, B pre-spermatogonia and C total germ cell number per mm² in the ex vivo cultured fetal testis tissue from GW 7–12 following treatment with flutamide (10⁻⁶ M) for 14 days. Gonocytes were determined as the number of OCT4⁺ cells per mm², pre-spermatogonia as the number of MAGE-A4⁺ cells per mm² and total germ cell number as the combined number of OCT4⁺ and MAGE-A4⁺ cells per mm². Values represent mean ± SEM, with N=5 (vehicle control), N=5 (flutamide). Significant difference compared to vehicle control *p<0.05