Skip to main content

Table 1 Antibodies, dilutions and retrieval buffers used

From: Identification of a window of androgen sensitivity for somatic cell function in human fetal testis cultured ex vivo

Antibody

Dilution

Retrieval buffer

Species

Supplier

Number

AR

1:75

TEG

Mouse

Santa Cruz

Sc-7305

BrdU

1:100

CIT

Mouse

Dako

M0744

CYP11A1

1:500

CIT

Rabbit

Sigma

HPA016436

cPARP

1:500

CIT

Rabbit

Cell Signaling

5625

MAGE-A4

1:250

TEG

Mouse

Gift from Prof. Spagnoli

NA

OCT4

1:250

TEG

Mouse

Santa Cruz

Sc-5279

SOX9

1:800

CIT

Rabbit

Millipore

AB5535

  1. Antigen retrieval was conducted by microwaving the sections in the indicated retrieval buffer for 1 min at 750W and 15 min at 350W. TEG buffer: 10 mM Tris, 0.5 mM EGTA, pH 9.0; citrate (CIT) buffer: 10 mM, pH 6.0. NA not available