Maternal blood pressure associates with placental DNA methylation both directly and through alterations in cell-type composition

Table 2 Placental CpGs previously found significantly associated with maternal SBP and DBP during pregnancy which were replicated in our study

						Associated BP indicators in our study
CpG	Position	Gene	Phenotype^a	Trimester	Direction of Effects^b	Early pregnancy	Late pregnancy	Pregnancy
cg05130406	chr12:75723912	CAPS2	SBP+DBP	3rd	1/1	-	SBP^c; MAP^c	SBP^c
cg10752545	chr11:19734701	NAV2	SBP+DBP	3rd	1/1	-	DBP; MAP^c	-
cg13534424	chr7:44365292	CAMK2B	SBP	1st and 3rd	1/1	SBP; DBP; MAP	SBP; DBP; MAP	SBP; DBP; MAP
cg23314283	chr13:53226751	SUGT1	SBP	1st	1/1	SBP; DBP; MAP	SBP; DBP; MAP	SBP; DBP; MAP
cg06880028	chr6:75918165	Intergenic	SBP+DBP	1st	1/1	-	-	PP; SBP^d
cg02918224	chr20:33298052	TP53INP2	SBP+DBP	3rd; 2nd; and 3rd	-1/1	-	SBP; MAP^c, PP^d	SBP; MAP^c

All 6 CpGs were replicated when further adjusting for cell-type composition and mediation analyses found no significant indirect effect of cell-type composition for theses CpGs
^aPhenotypes CpG methylation was found associated with in the EWAS by Workalemahu et al. [16]
^bDirection of effect observed in the study by Workalemahu et al. versus direction of effect observed in our study
^cSignificance was not reached anymore after excluding participants with preeclampsia
^dSignificance was reached only after excluding participants with preeclampsia

ISSN: 1741-7015