Fig. 2

Evaluation of the therapeutic effect of LD-CD mixture on the PD model. A–C Detection of the locomotor capacity of day 5 of the 5th instar larvae treated by MPTP (125 μM) with LD-CD mixture (0, 4, 8, and 20 mM; n = 5/locomotor situation). D Determination of dopamine content in PD silkworm brains with LD-CD mixture by UPLC (0, 4, and 8 mM; n = 4/concentration). E Detection of TH protein expressions by immunofluorescence among untreated silkworm brains, MPTP-treated (125 μM) silkworm brains, and brains of silkworms treated with MPTP (125 μM) and the LD-CD mixture (8 mM). F Determination of BmTH activity by enzyme-linked immunosorbent assay in PD silkworm brains with LD-CD mixture (0, 4, and 8 mM). G, H Detection of relative levels of BmDDC and BmPARK2 expression by RT-qPCR in brains of silkworms fed with the LD-CD mixture (0, 4, and 8 mM; n = 3/concentration). I Detection of total antioxidant capacity in silkworms fed with the LD-CD mixture (0, 4, and 8 mM; n = 3/concentration). All the experiments were performed at least 3 times and showed a similar pattern. Error bars depicted the mean with SD and were analysed using the Student’s t-test. The “ns” is an abbreviation for “no significance”: *p < 0.05, **p < 0.01, ***p < 0.001